SINGLE PARTICLE CRYO-ELECTRON MICROSCOPY
Join CEBEM’s regional school and discover the future of Microscopy!
Learn the foundational aspects of this technique, in a unique course where theory meets practice under the mentorship of international experts. Gain valuable insights for accessing this technology from South America.
Target audience:
doctoral or postdoctoral students
Enrollment capacity:
20 students. The course includes practical modules, hence there are limited positions.
Fellowships:
we will grant scholarships to cover the transportation and accommodation of selected students who do not reside in Rosario, Argentina. A scientific committee will select the candidates from all the received applications.
Application deadline:
April 12th
Program:
- Sample preparation and quality check
- Grid preparation and sample vitrification
- The electron microscope and image processing workflow
- Negative staining (NS)
- NS screening at the microscope
- Image quality and pre-processing
- Pre-processing
- Data processing: from 2D images to 3D maps
- Fitting, building and model refinement
- CryoEM sample screening and acquisition
- How to access from Argentina
- Advanced strategies (real life cryoEM)
- Validation and deposit
Tutors:
- Mario Borgnia - Director of the Cryo-EM Core at GISBL/DIR/NIEHS/NIH, USA
- Alejandro Buschiazzo - Director of the Molecular and Structural Microbiology Laboratory and the Protein Crystallography Unit at Institut Pasteur de Montevideo, Uruguay
- Rodrigo Portugal - Head of the Cryo-electron Microscopy and Single Particle Analysis facility at LNNano/CNPEM, Brazil
- Gabriela Dias Noske - LNNano/CNPEM, Brazil
- Mariana Araujo Ajalla - LNNano/CNPEM, Brazil
- Daniela Albanesi - Instituto de Biología Molecular y Celular de Rosario, Argentina.
- Lautaro Diacovich - Instituto de Biología Molecular y Celular de Rosario, Argentina.
- María Natalia Lisa - Instituto de Biología Molecular y Celular de Rosario, Argentina.
- Laura Buttigliero - Instituto de Física de Rosario.
- Lisandro Otero - Instituto de Biotecnología Ambiental y Salud, Universidad Nacional de Río Cuarto, Córdoba, Argentina. Centro de Re-diseño e Ingeniería de Proteínas, Universidad Nacional de San Martín, Buenos Aires, Argentina.
- Irina P. Suarez - Instituto de Biología Molecular y Celular de Rosario, Argentina.
- Mariano Dellarole - Instituto de Investigaciones en Bionanociencias (CIBION-CONICET).
CEBEM acknowledges support from the Chan Zuckerberg Initiative (grant “Building Bioimaging Capacity in South America”), aimed at integrating regional capacities in Structural Biology and Bioimaging, optimizing the use of sophisticated equipment, and disseminating integrative approaches.
